TaqMan® Mutation Detection Assays

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Use TaqMan® Mutation Detection Assays to determine the mutation status and percent mutation for a given sample by one of two experiment methods:

  1. Mutant allele assays paired with a gene reference assay
    2.
  2. Mutant allele assays paired with wild type allele assays
    3.
Learn more about the 3 types of assays. For additional information, please see our product information page.

Select a gene below to find your TaqMan® Mutation Detection Assay.
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Gene Symbol: KRAS
Entrez Gene ID: 3845
Transcript: NM_004985
Select All
 Assay Name Assay ID     Select All
 Assay Name Assay ID     COSMIC ID Nucleotide
Mutation		 Amino Acid
Change
KRAS_rf Hs00000174_rf
KRAS_516_mu Hs00000113_mu KRAS_516_wt Hs00000112_wt 516 c.34G>T p.G12C
KRAS_517_mu Hs00000115_mu KRAS_517_wt Hs00000114_wt 517 c.34G>A p.G12S
KRAS_518_mu Hs00000117_mu KRAS_518_wt Hs00000116_wt 518 c.34G>C p.G12R
KRAS_520_mu Hs00000119_mu KRAS_520_wt Hs00000118_wt 520 c.35G>T p.G12V
KRAS_521_mu Hs00000121_mu KRAS_521_wt Hs00000120_wt 521 c.35G>A p.G12D
KRAS_522_mu Hs00000123_mu KRAS_522_wt Hs00000122_wt 522 c.35G>C p.G12A
KRAS_527_mu Hs00000125_mu KRAS_527_wt Hs00000124_wt 527 c.37G>T p.G13C
KRAS_528_mu Hs00000127_mu KRAS_528_wt Hs00000126_wt 528 c.37G>A p.G13S
KRAS_529_mu Hs00000129_mu KRAS_529_wt Hs00000128_wt 529 c.37G>C p.G13R
KRAS_532_mu Hs00000131_mu KRAS_532_wt Hs00000130_wt 532 c.38G>A p.G13D
KRAS_552_mu Hs00000133_mu KRAS_552_wt Hs00000132_wt 552 c.182A>G p.Q61R
KRAS_553_mu Hs00000135_mu KRAS_553_wt Hs00000134_wt 553 c.182A>T p.Q61L
KRAS_554_mu Hs00000137_mu KRAS_554_wt Hs00000136_wt 554 c.183A>C p.Q61H
KRAS_555_mu Hs00000139_mu KRAS_555_wt Hs00000138_wt 555 c.183A>T p.Q61H
Gene Symbol: BRAF
Entrez Gene ID: 673
Transcript: NM_004333
Select All
 Assay Name Assay ID     Select All
 Assay Name Assay ID     COSMIC ID Nucleotide
Mutation		 Amino Acid
Change
BRAF_rf Hs00000172_rf
BRAF_476_mu Hs00000111_mu BRAF_476_wt Hs00000110_wt 476 c.1799T>A p.V600E
BRAF_467_mu Hs00000996_mu 467 c.1781A>G p.D594G
BRAF_473_mu Hs00000769_mu 473 c.1798_1799GT>AA p.V600K
BRAF_474_mu Hs00000770_mu 474 c.1798_1799GT>AG p.V600R
BRAF_475_mu Hs00000771_mu 475 c.1799_1800TG>AA p.V600E
BRAF_477_mu Hs00000772_mu 477 c.1799_1800TG>AT p.V600D
BRAF_478_mu Hs00000773_mu 478 c.1801A>G p.K601E
BRAF_1130_mu Hs00000839_mu 1130 c.1798G>A p.V600M
BRAF_18443_mu Hs00000957_mu 18443 c.1799T>C p.V600A
Gene Symbol: EGFR
Entrez Gene ID: 1956
Transcript: NM_005228
Select All
 Assay Name Assay ID     Select All
 Assay Name Assay ID     COSMIC ID Nucleotide
Mutation		 Amino Acid
Change
EGFR_rf Hs00000173_rf
EGFR_6213_mu Hs00000141_mu EGFR_6213_wt Hs00000140_wt 6213 c.2582T>A p.L861Q
EGFR_6224_mu Hs00000102_mu EGFR_6224_wt Hs00000142_wt 6224 c.2573T>G p.L858R
EGFR_6239_mu Hs00000104_mu EGFR_6239_wt Hs00000103_wt 6239 c.2156G>C p.G719A
EGFR_6240_mu Hs00000106_mu EGFR_6240_wt Hs00000105_wt 6240 c.2369C>T p.T790M
EGFR_6241_mu Hs00000108_mu EGFR_6241_wt Hs00000107_wt 6241 c.2303G>T p.S768I
EGFR_6252_mu Hs00000146_mu EGFR_6252_wt Hs00000109_wt 6252 c.2155G>A p.G719S
EGFR_6253_mu Hs00000148_mu EGFR_6253_wt Hs00000147_wt 6253 c.2155G>T p.G719C
EGFR_12376_mu* Hs00000241_mu NA NA 12376 c.2307_2308ins9 p.V769_D770insASV
EGFR_12377_mu Hs00000150_mu EGFR_12377_wt Hs00000149_wt 12377 c.2319_2320insCAC p.H773_V774insH
EGFR_12378_mu Hs00000152_mu EGFR_12378_wt Hs00000151_wt 12378 c.2310_2311insGGT p.D770_N771insG
EGFR_ex19dels_mu** Hs00000228_mu EGFR_ex19dels_wt Hs00000177_wt NA 19 deletions in EGFR exon19 NA
EGFR_6210_mu Hs00000153_mu 6210 c.2240_2251del12 p.L747_T751>S
EGFR_6218_mu Hs00000154_mu 6218 c.2239_2247del9 p.L747_E749del
EGFR_6220_mu Hs00000155_mu 6220 c.2238_2255del18 p.E746_S752>D
EGFR_6223_mu Hs00000156_mu 6223 c.2235_2249del15 p.E746_A750del
EGFR_6225_mu Hs00000157_mu 6225 c.2236_2250del15 p.E746_A750del
EGFR_6254_mu Hs00000158_mu 6254 c.2239_2253del15 p.L747_T751del
EGFR_6255_mu Hs00000159_mu 6255 c.2239_2256del18 p.L747_S752del
EGFR_12367_mu Hs00000160_mu 12367 c.2237_2254del18 p.E746_S752>A
EGFR_12369_mu Hs00000161_mu 12369 c.2240_2254del15 p.L747_T751del
EGFR_12370_mu Hs00000162_mu 12370 c.2240_2257del18 p.L747_P753>S
EGFR_12382_mu Hs00000163_mu 12382 c.2239_2248TTAAGAGAAG>C p.L747_A750>P
EGFR_12383_mu Hs00000164_mu 12383 c.2239_2251>C p.L747_T751>P
EGFR_12384_mu Hs00000165_mu 12384 c.2237_2255>T p.E746_S752>V
EGFR_12387_mu Hs00000166_mu 12387 c.2239_2258>CA p.L747_P753>Q
EGFR_12419_mu Hs00000167_mu 12419 c.2238_2252>GCA p.L747_T751>Q
EGFR_12422_mu Hs00000168_mu 12422 c.2238_2248>GC p.L747_A750>P
EGFR_12678_mu Hs00000169_mu 12678 c.2237_2251del15 p.E746_T751>A
EGFR_12728_mu Hs00000170_mu 12728 c.2236_2253del18 p.E746_T751del
EGFR_13551_mu Hs00000143_mu 13551 c.2235_2252>AAT p.E746_T751>I

* The EGFR_12376_mu assay does not have a corresponding wild type allele assay. Run EGFR_12376_mu with the EGFR_rf gene reference assay.

** The EGFR_ex19dels_mu assay detects 19 mutations 6210, 6218, 6220, 6223, 6225, 6254, 6255, 12367, 12369, 12370, 12382, 12383, 12384, 12387, 12419, 12422, 12678, 12728, 13551. Individual assays for each deletion mutation are also available. All EGFR exon 19 deletion assays can be run with either EGFR_rf gene reference assay or the EGFR_ex19dels_wt wild type allele assay.

There are 3 types of TaqMan® Mutation Detection Assays:

  • Mutant allele assay—detects specific or multiple mutant alleles. The assay name has the format: gene symbol_COSMIC ID_MU (e.g. KRAS_517_mu).
  • Wild type allele assay—detects a wild type allele. The assay name has the format: gene symbol_COSMIC ID_wt (e.g. KRAS_517_wt).
  • Gene reference assay—detects a mutation-free region of the gene within which the target mutations reside. The assay name has the format: gene symbol_rf (e.g. KRAS_rf).
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